New England Biolabs

New England Biolabs (NEB) is a privately owned biotechnology company founded in 1974 in Ipswich, Massachusetts, that has built its reputation on the discovery, production, and supply of restriction enzymes, DNA modifying enzymes, and molecular biology reagents. NEB's catalog of over 3,000 products is considered one of the most reliable in the field, and the company is respected for its scientific rigor and commitment to publishing research on its own products. The HiFi DNA Assembly (Gibson Assembly) reagents and the Q5 high-fidelity DNA polymerase are among their most widely adopted innovations, enabling seamless multi-fragment cloning and faithful PCR amplification. For next-generation sequencing library preparation, NEB's KAPA and Ultra library prep kits are trusted for their low bias and high efficiency. Their RNase H2-based PCR and cloning enzymes have found applications in synthetic biology workflows. NEB's catalog of glycobiology enzymes, including PNGase F for N-glycan release and a full suite of exoglycosidases, is unmatched for carbohydrate research. NEB also manufactures the Luna qPCR and RT-qPCR supermixes, epigenetic tools such as T4 DNA Methyltransferase, and the popular Monarch nucleic acid purification kits. Their active research program means new reagents are regularly derived from internal discovery, and their technical literature and protocols set the standard for the field.