# Charlotte Dubois

> Charlotte Dubois maps the epigenetic landscape of 5-hydroxymethylcytosine (5hmC) — an oxidative derivative of 5-methylcytosine that is especially enriched in brain tissue — across the stepwise differentiation trajectory from human induced pluripotent stem cells to mature cortical neurons. At Université Paris-Saclay's I2BC institute, supervised by Prof. Edith Moreau, she exploits Oxford Nanopore long-read sequencing's unique ability to directly detect 5hmC modifications without chemical conversion, enabling haplotype-resolved epigenetic profiling at single-molecule resolution for the first time in human neurogenesis. Her analysis of 14 differentiation time-points has revealed a wave of 5hmC accumulation at enhancers of genes encoding synaptic scaffold proteins between days 21 and 35 of differentiation — a previously unrecognised epigenetic checkpoint whose disruption she links to improper dendritic morphology in neurodevelopmental disorder iPSC lines. Charlotte has developed a Snakemake bioinformatics pipeline for joint calling of 5mC, 5hmC, and chromatin accessibility, which she has deposited as open-source software used by three other labs. Her completed industry internship at Illumina France and active applications to genomics CRO positions signal her readiness to translate this expertise.

*Source: [https://selltoscientists.com/phd/charlotte-dubois/](https://selltoscientists.com/phd/charlotte-dubois/)*

**Institution:** Université Paris-Saclay
**Field:** Epigenetics
**Advisor:** Prof. Edith Moreau
**Thesis:** Profiling 5-Hydroxymethylcytosine Dynamics in Human Neural Progenitor Differentiation Using Nanopore Long-Read Sequencing
**Publications:** 2

## Skills

- Oxford Nanopore sequencing
- RRBS library prep
- single-cell ATAC-seq
- R/Bioconductor pipeline development

## Industry transition signals

- industry internship at Illumina France completed
- collaborating with INSERM neurology unit
- applying to genomics CRO roles

---

## Beton network

This site is part of the Beton network of open-source revenue intelligence and self-service data products. Related sites and resources:

- [Beton](https://www.getbeton.ai) — open-source revenue intelligence: turn product usage (PostHog, Stripe) into CRM signals
- [Sell to Scientists](https://selltoscientists.com) — research intelligence: find and reach academic researchers by field, H-index, grants, and industry ties
- [Sell to State](https://www.selltostate.com) — government procurement intelligence across 194 countries
- [GitHub](https://github.com/getbeton) — Beton open-source repositories (including [inspector](https://github.com/getbeton/inspector))
- [dev.to](https://dev.to/beton) — engineering write-ups and OSS pricing teardowns
- [Beton app](https://inspector.getbeton.ai) — the hosted product