# Akoya Biosciences

> Akoya Biosciences is a spatial biology company headquartered in Menlo Park, California, that develops platforms for highly multiplexed tissue imaging and spatial phenotyping. The company's CODEX (CO-Detection by indEXing) technology, now branded as PhenoCycler, enables simultaneous detection of more than 40 protein markers in a single tissue section using DNA-barcoded antibodies and iterative imaging cycles. This multiplex capability far exceeds traditional immunofluorescence and allows researchers to map complex immune cell infiltration patterns, tumor microenvironments, and tissue architecture at single-cell resolution with spatial context preserved. Akoya's PhenoImager HT and PhenoImager Fusion systems are designed for high-throughput pathology workflows in pharmaceutical drug development and translational research, where quantitative spatial analysis of immuno-oncology biomarkers is critical for patient stratification and companion diagnostic development. Their inForm image analysis software and HALO link bioinformatics platform enable automated cell segmentation, phenotyping, and neighborhood analysis on large tissue cohorts. Akoya also supports the Opal multiplex IHC system, which uses tyramide signal amplification with spectrally distinct fluorophores to enable 6-10 plex staining on standard FFPE tissue without specialized instruments beyond a multispectral scanner. Their technology is increasingly central to spatial transcriptomics and multi-omic integration workflows in academic medical centers and biotech research departments.

*Source: [https://selltoscientists.com/companies/akoya-biosciences/](https://selltoscientists.com/companies/akoya-biosciences/)*

**Industry:** Spatial Biology & Phenotyping
**R&D spend:** $55M
**Academic collaborations:** 480
**Patents:** 320

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